《植物生理学报》 2014, 50(8): 1079-1088

蛋白质亚硝基化检测和鉴定方法的研究进展

汪达丽*, 张双双*, 刘建中**

浙江师范大学化学与生命科学学院, 浙江金华321004

通信作者： 刘建中；E-mail: jzliu@zjnu.cn；Tel: 0579-82288053

摘　要：

蛋白的亚硝基化是近期发现的一种类似于磷酸化、可逆的、不依赖于环磷酸鸟苷(cGMP)的一氧化氮修饰和调节蛋白功能的新途径。一经发现, 有关亚硝基化的研究呈指数级递增。亚硝基化参与从生长发育到抗病、抗逆等多个生理和病理过程。已有大量综述对亚硝基化调控蛋白功能从而影响某一生理生化及病理过程进行了总结。但迄今为止, 对检测蛋白亚硝基化的手段和鉴定亚硝基化位点的方法进行总结的文献综述仍屈指可数。据此, 我们对蛋白亚硝基化检测手段的发明、改进提高、亚硝基化位点的结构特点以及亚硝基化位点预测软件的开发等进行综述, 旨在为该领域内科研工作者提供方便。

关键词：亚硝基化; 一氧化氮; 生物素置换法; 翻译后修饰; LC-MS/MS

收稿：2014-03-25   修定：2014-05-16

资助：国家自然科学基金(31371407)、浙江省钱江人才计划(2013R10074)和浙江省自然科学基金(LY12C14001)。

Advances in Methodologies for Detection and Identification of the S-nitrosylated Proteins

WANG Da-Li*, ZHANG Shuang-Shuang*, LIU Jian-Zhong**

College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China

Corresponding author: WANG Da-Li; E-mail: jzliu@zjnu.cn; Tel: 0579-82288053

Abstract:

Similar to phosphorylation, S-nitrosylation is a newly identified protein modification in which nitric oxide (NO) moiety covalently attached to thiol group. S-nitrosylation is a reversible process and it can result in the alteration of protein functions in guanosine 3′, 5′-cyclic phosphate (cGMP) independent manner. It has been shown that S-nitrosylation plays important regulatory roles in various physiological and pathological processes from growth and development to biotic and abiotic stress responses. The research publications in this field have grown exponentially since its discovery. Up to date, numerous review articles have been published on how S-nitrosylation exerts its influences on protein functions. However, review articles that summarize the detection of S-nitrosylated proteins and the identification of the sites of this modification are rare. Thus, we reviewed the development, evolution and current status of the methodologies for protein S-nitrosylation studies.

Key words: S-nitrosolation; nitric oxide (NO); biotin switch assay (BSA); post-translational modification; LCMS/MS